Abstract Tumor hypoxia is an important factor for developing resistance to radiation therapy (RT) and presents a bleak prognosis in cancer patients undergoing treatment for RT resistant hepatocellular carcinoma.Here, we present the synthesis of liposome-coated Mn3O4 (MGN) nanoparticles (Lipo-MGN) and investigation of their therapeutic potential with RT utilizing CAMOMILE a HepG2 cancer model.According to in vitro research, Lipo-MGN effectively produced oxygen in the presence of H2O2 and significantly reduced the expression of HIF-1 in human HepG2 cells that were under hypoxic conditions.Lipo-MGN reversed the radio-resistance brought on by hypoxia and increased cell damage.
When Lipo-MGN and RT were administered together in a HepG2 xenograft mice model, the tumor growth was delayed more than with RT alone.As determined by MR imaging, liposome-MGN also exhibited Nappy Bin T1 contrast enhancement in tumor.According to these findings, Lipo-MGNs may increase the impact of RT by focusing tumor hypoxia.Hypoxic, radioresistant HepG2 cancer may be treated with Lipo-MGN in clinical studies.